Gas-liquid chromatography-mass spectrometry of synthetic ceramides

Gas--liquid chromatography-mass spectrometry of synthetic ceramides.

Two series of ceramides with either sphingosine (sphing-4-enine) or sphinganine as base and with one of the saturated fatty acids C(16), C(18), C(20), C(22), C(24), C(26), or oleic acid were analyzed as the 1,3-di-O-trimethylsilyl ether derivatives by gas chromatography-mass spectrometry. The fragments formed on electron impact can be divided into three main groups, namely "molecular weight fra...

Gas--liquid chromatography--mass spectrometry of synthetic ceramides containing 2-hydroxy acids.

Ceramides containing either sphingosine or sphinganine and one of the 2-hydroxy acids, 14h:0, 16h:0, 18h:0, 20h:0, 22h:0, 24h:0, and 26h:0 were prepared and separated by gas chromatography as the 1,3,2'-tri-O-tri-methylsilyl derivatives. Mass spectrometric analyses of these derivatives showed that the ions formed on electron impact can be used to determine unequivocally the structures of the lo...

Applications of Hadamard transform to gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry.

Successful application of the Hadamard transform (HT) technique to gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) is described. Novel sample injection devices were developed to achieve multiple sample injections in both GC and LC instruments. Air pressure was controlled by an electromagnetic valve in GC, while a syringe pump and Tee connector we...

Gas Chromatography - Mass Spectrometry

Gas Chromatography-Mass Spectrometry

Gas chromatography (GC) is a widely applied technique in many branches of science and technology. For over half a century, GC has played a fundamental role in determining how many components and in what proportion they exist in a mixture. However, the ability to establish the nature and chemical structure of these separated and quantified compounds is ambiguous and reduced, and requires a spect...